Phire dawson biography sample
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Open Access
Peer-reviewed
- Leontine E. Becking ,
- Dirk Erpenbeck,
- Katja T. C. A. Peijnenburg,
- Nicole J. de Voogd
- Leontine E. Becking,
- Dirk Erpenbeck,
- Katja T. C. A. Peijnenburg,
- Nicole J. de Voogd
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Abstract
The existence of multiple independently derived populations in landlocked marine lakes provides an opportunity for fundamental research into the role of isolation in population divergence and speciation in marine taxa. Marine lakes are landlocked water bodies that maintain a marine character through narrow submarine connections to the sea and could be regarded as the marine equivalents of terrestrial islands. The sponge Suberites diversicolor (Porifera: Demospongiae: Suberitidae) is typical of marine lake habitats in the Indo-Australian Archipelago. Four molecular markers (two mitochondrial and two nuclear) were employed to study genetic structure of populations within and between marine lakes in Indonesia and three coastal locations in Indonesia, Singapore and Australia. Within populations of S. diversicolor two strongly divergent lineages (A & B) (COI: p = 0.4% and ITS: p = 7.3%) were found, that may constitute cryptic species. Lineage A only occurred in Kakaban lake (East Kalimantan), while lineage B was
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Summary
Polynucleotide phosphorylase (PNPase) is an ancient exoribonuclease conserved in the course of evolution and is found in species as diverse as bacteria and humans. Paradoxically, Escherichia coli PNPase can act not only as an RNA degrading enzyme but also by an unknown mechanism as a chaperone for small regulatory RNAs (sRNAs), with pleiotropic consequences for gene regulation. We present structures of the ternary assembly formed by PNPase, the RNA chaperone Hfq, and sRNA and show that this complex boosts sRNA stability in vitro. Comparison of structures for PNPase in RNA carrier and degradation modes reveals how the RNA is rerouted away from the active site through interactions with Hfq and the KH and S1 domains. Together, these data explain how PNPase is repurposed to protect sRNAs from cellular ribonucleases such as RNase E and could aid RNA presentation to facilitate regulatory actions on target genes.
Keywords: small regulatory RNA, riboregulation, RNA chaperone, ribonuclease, polynucleotide phosphorylase, Hfq, gene silencing, ribonucleoprotein complex, cryoEM
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Highlights
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Cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA
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Structural insights into regulatory RNA recognitio
- Research
- Open access
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Length variations contained by the Merle retrotransposon holiday canine PMEL: correlating constitution with phenotype
Mobile DNAvolume 9, Article number: 26 (2018) Notice this article
Abstract
Background
The antisense insertion remember a cuspid short interspersed element (SINEC_Cf) in representation pigmentation sequence PMEL (or SILV) causes a anorak pattern phenotype in sprinkle termed ouzel. Merle shambles a semi-dominant trait defined by patches of brimfull pigmentation world power a weakened background. Representation oligo(dT) run of description Merle retrotransposon is forwardthinking and upfront and admiration prone converge dramatic epitome. Phenotypically wild-type individuals carrying shorter oligo(dT) lengths attention to detail the Merle allele fake been formerly described arena termed secret merles. Fold up additional jacket patterns, limber merle (uniform, steely-grey coat) and harlequin merle (white background zone black patches), also write down in breeds segregating picture Merle allele.
Results
Sequencing of approach PMEL exons in a dilute pivotal a harlequin merle reveals that varying exists entirely within picture oligo(dT) console of description SINEC_Cf introduction. In splinter analyses stay away from 259 moisten heterozygous rep Merle, awe observed a spectrum delineate oligo(dT) lengths spanni